Biochemistry Laboratory

An undergraduate laboratory course in biochemistry should aim to give students an introduction to as many of the important techniques currently employed by practicing biological chemists as is possible. However, it is recognized that not all such techniques can be covered in one course. Given below is first a list of general methods that should be represented in at least one experiment in a modern biochemistry laboratory course. However, a good course would also include experience with a few more specific techniques. Some typical examples from which a selection might be made are given in the second list.

Important General Techniques:

Error and statistical analysis of experimental data
Spectroscopic methods
Electrophoretic techniques
Chromatographic separations
Isolation and characterization of biological macromolecules

Some Selected Additional Techniques (not inclusive):

Safe use of radioisotopes
Enzyme Idnetics
Immunoassay methods
DNA cloning and sequencing
Plasmid isolation and mapping
Protein purification
Peptide sequencing
Computer graphics

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